Mchanganyiko Rahisi wa Kijani wa SYBR RT-qPCR
Utangulizi
Rahisi sana SYBR Green RT-qPCR Master Mix(yenye dNTPs, lyophilized) ni mchanganyiko tayari kutumika, lyophilizedmaster kwa ajili ya ukuzaji wa ubora wa juu wa RT-qPCR ambao unaweza kutumika kugundua DNA au sampuli za RNA kwa haraka.Themixin ni pamoja na Super HP Taq DNA polymerase iliyozuiwa mara mbili, M-MLV reverse transcriptase (RNaseH-), MgCl2, SYBR rangi ya kijani na dNTPs.Unda upya mchanganyiko mkuu kwa kuongeza maji ya kiwango cha PCR pamoja na kiolezo chako , Taqman huchunguza na vianzio kwa jumla ya ujazo wa 20 µl.
Utendaji• Usahihi: Hatari ndogo ya kuambukizwa
•Unyeti wa hali ya juu: Utendaji bora katika umakinifu wa violezo vya chini.
•Urahisi: Imechanganywa awali na ni bure kutumia.
Hatua za uendeshaji
| PAKA nambari. | Sehemu | Vipimo | Kiasi | Kumbuka |
| KY135-01 | Rahisi sana SYBR Green RT-qPCR Master Mix (pamoja na dNTPs, lyophilized) | 48T/kit | Mirija 48 | Vipande 8 vya visima, 0.1mL |
| Maji ya daraja la PCR | 1.5mL/Tube | 1Tube | Cryotube, 2.0 mL | |
| KY135-02 | Rahisi sana SYBR Green RT-qPCR Master Mix (pamoja na dNTPs, lyophilized) | 48T/kit | Mirija 48 | Vipande 8 vya visima, 0.2mL |
| Maji ya daraja la PCR | 1.5mL/Tube | 1Tube | Cryotube, 2.0 mL | |
| KY135-03 | Rahisi sana SYBR Green RT-qPCR Master Mix (pamoja na dNTPs, lyophilized) | 48T/kit | 2Tubes | Cryotube, 2.0 mL |
| Maji ya daraja la PCR | 1.5mL/Tube | 1Tube | Cryotube, 2.0 mL | |
| KY135-04 | Rahisi sana SYBR Green RT-qPCR Master Mix (pamoja na dNTPs, lyophilized) | 500T/kit | 1Tube | / |
| Maji ya daraja la PCR | 10 ml | Chupa 1 | / |
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